Activity-based MET G1163R kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
MET G1163R
Enzyme
Human MET G1163R, mutant
HGNC
MET (G1163R)
Manning ID
MET
Synonym
MET, HGFR, RCCP2, c-MET, MET G1163R, c-MET (G1163R)
UniProt
P08581 (MET_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
Poly(Ala,Glu,Lys,Tyr)6:2:5:1, ATP
Reaction
Poly(Ala,Glu,Lys,Tyr)6:2:5:1 + 33P-ATP = 33P-Poly(Ala,Glu,Lys,Tyr)6:2:5:1 + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 2700 |
| Sorafenib | 10000 |
| Sunitinib | 41000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | MET G1163R |
| 355 wt Profiler | No |
| 355 IC50 wt Profiler | No |
| Mutant Profiler Addon | Yes |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – MET G1163R (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 1650-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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