Activity-based MET L1195V kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
MET L1195V
Enzyme
Human MET L1195V, mutant
HGNC
MET (L1195V)
Manning ID
MET
Synonym
MET, HGFR, RCCP2, MET L1195V
UniProt
P08581 (MET_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
Poly(Ala,Glu,Lys,Tyr)6:2:5:1, ATP
Reaction
Poly(Ala,Glu,Lys,Tyr)6:2:5:1 + 33P-ATP = 33P-Poly(Ala,Glu,Lys,Tyr)6:2:5:1 + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1100 |
| Sorafenib | 10000 |
| Sunitinb | 100000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | MET L1195V |
| 345 wt Profiler | No |
| 345 IC50 wt Profiler | No |
| Mutant Profiler Addon | Yes |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – MET L1195V |
| Product Number |
1651-0000-1 |
| Web Shop | Reaction Biology Online Shop |
PDF