Activity-based MET M1250T kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
MET M1250T
Enzyme
Human MET M1250T, mutant
HGNC
MET (M1250T)
Manning ID
MET
Synonym
MET, HGFR, RCCP2, c-MET, MET M1250T, c-MET (M1250T)
UniProt
P08581 (MET_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
Poly(Ala,Glu,Lys,Tyr)6:2:5:1, ATP
Reaction
Poly(Ala,Glu,Lys,Tyr)6:2:5:1 + 33P-ATP = 33P-Poly(Ala,Glu,Lys,Tyr)6:2:5:1 + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1223 |
| Sorafenib | 10000 |
| Sunitinib | 27000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | MET M1250T |
| 355 wt Profiler | No |
| 355 IC50 wt Profiler | No |
| Mutant Profiler Addon | Yes |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | IC50 MET Profiler |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – MET M1250T (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0982-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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