Activity-based MINK/MINK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MINK/MINK1
Enzyme
Human MINK/MINK1
HGNC
MINK1
Synonym
B55, YSK2, MAP4K6, ZC3
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1.3 |
| Ro 31-8220 | 37 |
| GW 5074 | 39 |
| H-89 | 12,000 |
Scientific Information
Q8N4C8 (http://www.uniprot.org/uniprot/Q8N4C8)
Screening Location
Malvern, USA
Reference compound IC50 for MINK/MINK1
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