Activity-based MKK4 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
MKK4
Enzyme
Human MKK4, wt
HGNC
MAP2K4
Manning ID
MAP2K4
Synonym
JNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SEK1, SERK, SAPK, MAP2K4, PRKMK4, SEK, SERK1
UniProt
P45985 (MP2K4_HUMAN)
Kinase Type
Protein kinase
Kinase Group
STE
Assay Properties
Assay Format
33PanQinaseTM
Substrates
JNK1 KRKR, ATP
Reaction
JNK1 KRKR + 33P-ATP = 33P-JNK1 KRKR + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 230 |
| Sorafenib | 10000 |
| Sunitinb | 100000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | MKK4 |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – MKK4 |
| Product Number | 0948-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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