Activity-based MKK4 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MKK4
Enzyme
Human MKK4
HGNC
MAP2K4
Synonym
Dual specificity mitogen-activated protein kinase kinase 4, JNK-activating kinase 1, MAPK/ERK kinase 4, MEK4, SAPK/ERK kinase 1, SEK1, JNKK1, SAPK1, MAP2K4, MP2K4
Scientific Information
P45985 (http://www.uniprot.org/uniprot/P45985)
Assay Properties
Substrates
JNK1 (K55M), 5 µM
ATP, 10 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 3,100 |
| Ro 31-8220 | 12,000 |
| GW5074 | 3,400 |
| H-89 | 25,900 |
| Rottlerin | >100,000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for MKK4
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