Activity-based MKK6 SDTD kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
MKK6 SDTD
Enzyme
Human MKK6 SDTD, wt
HGNC
MAP2K6 (S207D/T211D)
Manning ID
MAP2K6
Synonym
MAPKK6, MEK6, MKK6, PRKMK6, SAPKK3, MAP2K6, MKK6 S207D/T211D, MKK6 SDTD
UniProt
P52564 (MP2K6_HUMAN)
Kinase Type
Protein kinase
Kinase Group
STE
Assay Properties
Assay Format
33PanQinaseTM
Substrates
p38-alpha KA, ATP
Reaction
p38-alpha KA + 33P-ATP = 33P-p38-alpha KA + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 130 |
| Sorafenib | 10000 |
| Sunitinib | 24000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | MKK6 SDTD |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – MKK6 SDTD (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0396-0000-1 |
| Web Shop | Reaction Biology Online Shop |
PDF