Activity-based MKK7 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
MKK7
Enzyme
Human MKK7, wt
HGNC
MAP2K7
Manning ID
MAP2K7
Synonym
Jnkk2, MAPKK7, MKK7, PRKMK7, MAP2K7, MEK7
UniProt
O14733 (MP2K7_HUMAN)
Kinase Type
Protein kinase
Kinase Group
STE
Assay Properties
Assay Format
33PanQinaseTM
Substrates
JNK1 KRKR, ATP
Reaction
JNK1 KRKR + 33P-ATP = 33P-JNK1 KRKR + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 740 |
| Sorafenib | 10000 |
| Sunitinb | 100000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | MKK7 |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – MKK7 |
| Product Number | 0726-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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