Activity-based MKK7 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MKK7
Enzyme
Human MKK7
HGNC
MAP2K7
Synonym
MAPKK7, MEK7, PRKMK7
Substrates
JNK (K55M), 3 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 440 |
| Ro 31-8220 | 25,000 |
| GW 5074 | 28,000 |
| H-89 | >50,000 |
| Rottlerin | >50,000 |
Scientific Information
O14733 (http://www.uniprot.org/uniprot/O14733)
Screening Location
Malvern, USA
Reference compound IC50 for MKK7
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