Activity-based MLK2/MAP3K10 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MLK2
Enzyme
Human MLK2
HGNC
MAP3K10
Synonym
MLK2, MST, MEKK10
Substrates
MBP (Myelin Basic Protein), 10 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1.5 |
| Ro 31-8220 | 260 |
| GW 5074 | 3,200 |
| H-89 | 29,000 |
| Rottlerin | 32,800 |
Scientific Information
Q02779 (http://www.uniprot.org/uniprot/Q02779)
Screening Location
Malvern, USA
Reference compound IC50 for MLK2/MAP3K10
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