Activity-based MLK3/MAP3K11 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MLK3
Enzyme
Human MLK3
HGNC
MAP3K11
Synonym
MGC17114, MLK-3, PTK1, SPRK
Substrates
MBP (Myelin Basic Protein), 10 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 0.54 |
| Ro 31-8220 | 60 |
| GW 5074 | 308 |
| H-89 | 27,500 |
| Rottlerin | 14,000 |
Scientific Information
Q16584 (http://www.uniprot.org/uniprot/Q16584)
Screening Location
Malvern, USA
Reference compound IC50 for MLK3/MAP3K11
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