Activity-based MLK4 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MLK4
Enzyme
Human MLK4
HGNC
MAP3K21
Synonym
KIAA1804
Substrates
MEK1 (K97R), 5 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1,600 |
| Ro 31-8220 | 210 |
| GW 5074 | >50,000 |
| H-89 | >50,000 |
| Rottlerin | 14,000 |
Scientific Information
Q5TCX8 (http://www.uniprot.org/uniprot/Q5TCX8)
Screening Location
Malvern, USA
Reference compound IC50 for MLK4
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