MMP 12 Protease Assay Service

MMP 12 Protease Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview

CAT#
MMP12

Enzyme
MMP-12, catalytic domain from human recombinant (residues 84-255) expressed in E. coli with C-terminal tag

Synonym
HME, Matrix metalloproteinase-12, MMP-12, Macrophage elastase, ME

Substrates
(5-FAM/QXLTM) FRET peptide

Assay Properties

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (M)
NNGH 2.04E-09
Actinonin 1.36E-07
GM6001 3.07E-10
MMP Inhibitor III 2.35E-09
MMP-9/MMP-13 Inhibitor I 1.41E-09
Scientific Information
P39900 (http://www.uniprot.org/uniprot/P39900)

Screening Location
Malvern, USA

Reference compound IC50 for MMP-12
Reference compound IC50 for MMP-12