MMP 12 Protease Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
MMP12
Enzyme
MMP-12, catalytic domain from human recombinant (residues 84-255) expressed in E. coli with C-terminal tag
Synonym
HME, Matrix metalloproteinase-12, MMP-12, Macrophage elastase, ME
Substrates
(5-FAM/QXLTM) FRET peptide
Assay Properties
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (M) |
|---|---|
| NNGH | 2.04E-09 |
| Actinonin | 1.36E-07 |
| GM6001 | 3.07E-10 |
| MMP Inhibitor III | 2.35E-09 |
| MMP-9/MMP-13 Inhibitor I | 1.41E-09 |
Scientific Information
P39900 (http://www.uniprot.org/uniprot/P39900)
Screening Location
Malvern, USA
Reference compound IC50 for MMP-12
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