MMP 13 Protease Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
MMP13
Enzyme
MMP-13, catalytic domain from human recombinant (residues 85-255) expressed in E. coli with C-terminal tag
Synonym
Matrix metalloproteinase-13, MMP-13
Substrates
(5-FAM/QXLTM) FRET peptide
Assay Properties
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (M) |
|---|---|
| NNGH | 2.57E-09 |
| Actinonin | 2.16E-08 |
| GM6001 | 1.13E-10 |
| MMP Inhibitor III | 4.40E-10 |
| MMP-9/MMP-13 Inhibitor I | 7.22E-10 |
Scientific Information
P45452 (http://www.uniprot.org/uniprot/P45452)
Screening Location
Malvern, USA
Reference compound IC50 for MMP-13
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