MMP 14 Protease Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
MMP14
Enzyme
MMP-14, catalytic domain from human recombinant (residues Tyr92-Arg278) expressed in E. coli with C-terminal tag
Synonym
MMP-14, Membrane-type matrix metalloproteinase 1, MT-MMP 1, MTMMP1, Membrane-type-1 matrix metalloproteinase, MT1-MMP, MT1MMP, MMP-X1
Substrates
(5-FAM/QXLTM) FRET peptide
Assay Properties
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (M) |
|---|---|
| NNGH | 2.53E-09 |
| Actinonin | 7.93E-07 |
| GM6001 | 4.71E-10 |
| MMP Inhibitor III | 3.70E-09 |
| MMP-9/MMP-13 Inhibitor I | 4.19E-09 |
Scientific Information
P50281 (http://www.uniprot.org/uniprot/P50281)
Screening Location
Malvern, USA
Reference compound IC50 for MMP-14
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