MMP 3 Protease Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
MMP3
Enzyme
MMP-3, catalytic domain from human recombinant (residues 83-255) in E. coli with C-terminal tag
Synonym
Matrix metalloproteinase-3, MMP-3, Transin-1, SL-1
Substrates
(5-FAM/QXLTM) FRET peptide
Assay Properties
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (M) |
|---|---|
| NNGH | 1.65E-08 |
| Actinonin | 7.23E-07 |
| GM6001 | 1.80E-8 |
| MMP-9/MMP-13 Inhibitor I | 1.41E-08 |
| MMP-2/MMP-3 Inhibitor I | 5.16E-06 |
Scientific Information
P08254 (http://www.uniprot.org/uniprot/P08254)
Screening Location
Malvern, USA
Reference compound IC50 for MMP-3
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