Activity-based MNK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MNK2
Enzyme
Human MNK2
HGNC
MKNK2
Synonym
MNK2
Substrates
MBP (Myelin Basic Protein), 10 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 15.5 |
| Ro 31-8220 | 14,600 |
| GW 5074 | 3,700 |
| H-89 | 19,300 |
| Rottlerin | 20,000 |
Scientific Information
Q9HBH9 (http://www.uniprot.org/uniprot/Q9HBH9)
Screening Location
Malvern, USA
Reference compound IC50 for MNK2/MKNK2
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