MOP (μ opioid) Biochemical Binding Assay for compound screening and profiling via competitive radioligand binding.
Target Overview
CAT#
MOP Binding
Synonyms
OPRM1, Mu-type opioid receptor mu 1
GPCR Target
Human MOP
Source
Membrane preparation from HEK293 cells stably transfected with human MOP receptor.
Scientific Information
OPRM (https://www.uniprot.org/uniprotkb/P35372/entry)
Assay Properties
Protocol
Radioligand filter binding to MOP membrane preparation ± cold competing ligand; radiometric measurement on MicroBeta scintillation counter
Readout
CPM representing 3H-disintegration detected using MicroScint-20
Reference Compound IC50
| Compound IC50 (nM) | Radioligand (nM) |
|---|---|
| DAMGO (8.6) | DAMGO (15) |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website In Vitro Safety Pharmacology Profiling
Reference compound binding data for MOP
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