MTHFD1 Replication-related Assay for compound screening and profiling via quantification of enzyme activity.
Target Overview
CAT#
MTHFD1
Enzyme
Human C-1-tetrahydrofolate synthase, cytoplasmic
Synonyms
C1-THF synthase, Methylenetetrahydrofolate dehydrogenase, Epididymis secretory sperm binding protein, MTHFC, MTHFD
Substrate
50 µM Folitixorin (5,10-Methylenetetrahydrofolic Acid), 100 µM NADP
Assay Properties
Reaction
(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate+ NADP = 5,10-methenyltetrahydrofolate + NADPH
End point measurement, Diaphorase/Resazurin coupled detection, Resorufin fluorescence measurement
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| DS18561882 | 0.41 |
| DS44960156 | 29.8 |
Scientific Information
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Replication-related Assays
Reference compound IC50 for MTHFD1
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