mTOR/FRAP1 Kinase Assay Service

Activity-based mTOR/FRAP1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
mTOR

Enzyme
Human mTOR

HGNC
MTOR

Synonym
FRAP1, FRAP, FRAP2, RAFT1, RAPT1

Substrates
4EBP1 1 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
LY294002 1,030
Wortmannin 727
PI3Kγ Inhibitor 952
PI-103 31.5
Rapamycin 77,000
Rapamycin + FKBP12 56
Scientific Information
P42345 (http://www.uniprot.org/uniprot/P42345)

Screening Location
Malvern, USA

Reference compound IC50 for mTOR
Reference compound IC50 for mTOR

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