Activity-based MUSK kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MUSK
Enzyme
Human MUSK
HGNC
MUSK
Synonym
MUSK
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 2.2 |
| PP2 | >100,000 |
| AG1478 | 9,200 |
| EGFR/Erb Inhibitor | >100,000 |
| D-64406 | 560 |
Scientific Information
O15146 (http://www.uniprot.org/uniprot/O15146)
Screening Location
Malvern, USA
Reference compound IC50 for MUSK
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