Activity-based MYLK2 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
MYLK2
Enzyme
Human MYLK2, wt
HGNC
MYLK2
Manning ID
skMLCK
Synonym
KMLC, MLCK, MYLK2, skMLCK, MLCK2, MLCK2/MYLK2
UniProt
Q9H1R3 (MYLK2_HUMAN)
Kinase Type
Protein kinase
Kinase Group
CAMK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RB-S6P, ATP
Reaction
RB-S6P + 33P-ATP = 33P-RB-S6P + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 107 |
| Sorafenib | 4800 |
| Sunitinb | 50 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | MYLK2 |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – MYLK2 |
| Product Number |
0445-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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