MYLK3 Kinase Assay Service

Activity-based MYLK3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
MYLK3

Enzyme
Human MYLK3

HGNC
MYLK3

Synonym
caMLCK, MLCK

Substrates
Peptide substrate, [KKRPQRRYSNVF], 20 µM
Ca2+-Calmodulin, 1 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 130
Ro 31-8220 41,000
GW 5074 1,300
H-89 >100,000
Rottlerin >100,000
Scientific Information
Q32MK0 (http://www.uniprot.org/uniprot/Q32MK0)

Screening Location
Malvern, USA

Reference compound IC50 for MYLK3
Reference compound IC50 for MYLK3

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