MYSM1 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
MYSM1
Enzyme
Human MYSM1
Synonym
KIAA1915, 2A-DUB, Myb-like, SWIRM and MPN domain-containing protein 1
Scientific Information
Q5VVJ2 (https://www.uniprot.org/uniprotkb/Q5VVJ2/entry)
Assay Properties
Substrate
100 nM Ubiquitin Rhodamine 110 Protein
Reaction
Ubiquitin Rhodamine 110 = Ubiquitin + Rhodamine 110
Rhodamine 110 fluorescence, kinetic measurement
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| UCH-L1 Inhibitor | 16.8 |
| UCH-L3 Inhibitor | 26.8 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Deubiquitination Assays (DUB assays)
Reference Compound IC50 for MYSM1
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