Nicotinic acetylcholine receptor (nAChR) α4β2 Functional Cell-based Antagonist Assay for compound screening and profiling via fluorescence-based quantification of intracellular calcium.
Target Overview
CAT#
nAChR α4β2 Functional Antagonist
Synonyms
CHRNA4/CHRNB2, Alpha-4 beta-2 nicotinic receptor
GPCR Target
Human nAChR (α4β2)
Cell Line
CHO-K1/OPRM1/Gα15
Scientific Information
Assay Properties
Protocol
30 min incubation with antagonist; nAChR α4β2 activation by agonist application using FLIPR Penta system cells treated with Calcium 6 dye; fluorescence kinetic measurement
Readout
Calcium 6 dye fluorescence tracking changes in intracellular calcium
Reference Compound IC50
| Compound IC50 (nM) | Agonist (nM) |
|---|---|
| Mepyramine (21) | Epibatidine (300) |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website In Vitro Safety Pharmacology Profiling
Reference compound inhibition of nAChR α4β2
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