Nav1.5 (Cardiac voltage-gated sodium channel) Manual Patch Clamp InVEST44 Assay for compound screening and profiling via luminescence recordings.
Target Overview
CAT#
Nav1.5 MPC InVEST44
GPCR Target
Human Nav1.5
Cell Line
HEK293 cells overexpressing human Nav1.5
Scientific Information
SCN5A (https://www.uniprot.org/uniprotkb/Q14524/entry)
Assay Properties
Protocol
Individual cells expressing Nav1.5 studied using whole-cell manual patch electrophysiology; compounds bath applied using constant gravity perfusion for 2-3 min or until steady state current reached
Readout
Maximum negative current (pA) elicited by a -10 mV step from -120 mV
Reference Compound IC50
| Compound IC50 (nM) |
|---|
| TTX (1104) |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website In Vitro Safety Pharmacology Profiling
Reference compound inhibition of Nav1.5
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