Activity-based NEK1 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
NEK1
Enzyme
Human NEK1, wt
HGNC
NEK1
Manning ID
NEK1
Synonym
KIAA1901, NY-REN-55, NEK1, NY-REN-55
UniProt
Q96PY6 (NEK1_HUMAN)
Kinase Type
Protein kinase
Kinase Group
Other
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-CHKtide, ATP
Reaction
RBER-CHKtide + 33P-ATP = 33P-RBER-CHKtide + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 470 |
| Sorafenib | 10000 |
| Sunitinb | 100000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | NEK1 |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – NEK1 |
| Product Number |
0844-0000-1 |
| Web Shop | Reaction Biology Online Shop |
PDF