Activity-based NEK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
NEK1
Enzyme
Human NEK1
HGNC
NEK1
Synonym
KIAA1901, NY-REN-55,
Substrates
MBP (Myelin Basic Protein), 10 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 18.7 |
| Ro 31-8220 | 7,700 |
| GW 5074 | 24,700 |
| H-89 | 47,000 |
Scientific Information
Q96PY6 (http://www.uniprot.org/uniprot/Q96PY6)
Screening Location
Malvern, USA
Reference compound IC50 for NEK1
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