Activity-based NEK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
NEK2
Enzyme
Human NEK2
HGNC
NEK2
Synonym
NLK1, HsPK21, HsPK 21
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 650 |
| Ro 31-8220 | >20,000 |
| GW 5074 | 7000 |
| Rottlerin | 8,000 |
Scientific Information
P51955 (http://www.uniprot.org/uniprot/P51955)
Screening Location
Malvern, USA
Reference compound IC50 for NEK2
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