NEK6 Kinase Assay Service

Activity-based NEK6 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
NEK6

Enzyme
Human NEK6

HGNC
NEK6

Synonym
SID6-1512

Substrates
Casein, 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine >100,000
Ro 31-8220 >100,000
GW 5074 19,000
H-89 >100,000
PKR Inhibitor 12,300
Scientific Information
Q9HC98 (http://www.uniprot.org/uniprot/Q9HC98)

Screening Location
Malvern, USA

Reference compound IC50 for NEK6
Reference compound IC50 for NEK6

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