Activity-based NEK7 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
NEK7
Enzyme
Human NEK7
HGNC
NEK7
Synonym
NEK7
Substrates
Casein, 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 33,000 |
| Ro 31-8220 | >100,000 |
| GW 5074 | 47,000 |
| H-89 | >100,000 |
| PKR Inhibitor | 11,000 |
Scientific Information
Q8TDX7 (http://www.uniprot.org/uniprot/Q8TDX7)
Screening Location
Malvern, USA
Reference compound IC50 for NEK7
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