Activity-based NEK9 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
NEK9
Enzyme
Human NEK9
HGNC
NEK9
Synonym
DKFZp434D0935, MGC16714, NERCC,
Substrates
MBP (Myelin Basic Protein), 10 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 306 |
| Ro 31-8220 | 18,200 |
| GW5074 | 8,700 |
| Rottlerin | 148,000 |
Scientific Information
Q8TD19 (http://www.uniprot.org/uniprot/Q8TD19)
Screening Location
Malvern, USA
Reference compound IC50 for NEK9
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