Norepinephrine transporter (NET) Biochemical Binding Assay for compound screening and profiling via competitive radioligand binding.
Target Overview
CAT#
NET Binding
GPCR Target
Human NET
Source
Membrane preparation from MDCK cells stably transfected with human NET receptor
Scientific Information
NET (https://www.uniprot.org/uniprotkb/Q71UR5/entry)
Assay Properties
Protocol
Radioligand filter binding to NET membrane preparation ± cold competing ligand; radiometric measurement on MicroBeta scintillation counter
Readout
CPM representing 3H-disintegration detected using MicroScint-20
Reference Compound IC50
| Compound IC50 (nM) | Radioligand (nM) |
|---|---|
| Nisoxetine (1.1) | Nisoxetine (4) |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website In Vitro Safety Pharmacology Profiling
Reference compound binding data for NET
PDF