Activity-based NIK kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
NIK
Enzyme
Human NIK, wt
HGNC
MAP3K14
Manning ID
NIK
Synonym
FTDCR1B, HS, HSNIK, MAP3K14, NIK, NIK/MAP3K14
UniProt
Q99558 (M3K14_HUMAN)
Kinase Type
Protein kinase
Kinase Group
STE
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-CHKtide, ATP
Reaction
RBER-CHKtide + 33P-ATP = 33P-RBER-CHKtide + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 2433 |
| Sorafenib | 10000 |
| Sunitinb | 84000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | NIK |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – NIK |
| Product Number | 0344-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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