NLK Kinase Assay Service

Activity-based NLK kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
NLK

Enzyme
Human NLK

HGNC
NLK

Synonym
NLK

Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 86
Ro 31-8220 4,600
GW 5074 490
H-89 23,000
Rottlerin 14,000
Scientific Information
Q9UBE8 (http://www.uniprot.org/uniprot/Q9UBE8)

Screening Location
Malvern, USA

Reference compound IC50 for NLK
Reference compound IC50 for NLK

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