NQO1 NAD(P)H Dehydrogenase Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
NQO1
Enzyme
Human NQO1
Substrate
5 µM resazurin, 20 µM NADH
Assay Properties
Reaction
Resazurin + NADH = Resorufin + NAD
Resorufin fluorescence, kinetic measurement
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Dicoumarol | 5.6 |
Scientific Information
Reference compound inhibition of NQO1
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