Activity-based NRMT1 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
NRMT1
Enzyme
Human NRMT1
Synonym
N-terminal Xaa-Pro-Lys N-methyltransferase 1, NTMT1, N-terminal RCC1 methyltransferase, Methyltransferase-like protein 11A, METTL11A
Substrates
RCC1, 5 µM
SAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + N-terminal-A/S/PPK-protein = S-adenosyl-L-homocysteine + protein [methyl-³H]-L- lysine
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| SAH | 3,300 |
| Chaetocin | >100,000 |
| Sinefungin | 6,900 |
| Suramin | >100,000 |
Scientific Information
Q9BV86 (http://www.uniprot.org/uniprot/Q9BV86)
Screening Location
Malvern, USA
Reference compound IC50 for NRMT1
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