Activity-based NSD2 (E1099K) Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
NSD2 (E1099K)
Enzyme
Human NSD2 (E1099K)
Synonym
Probable histone-lysine N-methyltransferase NSD2, Multiple myeloma SET domain-containing protein Nuclear SET domain-containing protein 2, Protein trithorax-5, Wolf-Hirschhorn syndrome candidate 1 protein, WHSC1, KIAA1090, MMSET, TRX5
Substrates
Nucleosomes, 0.05 mg/ml
SAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + histone L- lysine = S-adenosyl-L-homocysteine + histone [methyl-³H]-L- lysine
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| SAH | 2,000 |
| Sinefugin | >100,000 |
| BIX01294 | >100,000 |
| Chaetocin | 2,100 |
Scientific Information
O96028 (http://www.uniprot.org/uniprot/O96028)
Screening Location
Malvern, USA
Reference compound IC50 for NSD2 (E1099K)
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