Activity-based p300 Histone Acetyltransferase Assay for compound screening and profiling via radiometric HAT assay.
Target Overview
CAT#
p300
Enzyme
Human p300
Synonyms
Histone acetyltransferase p300, E1A-associated protein p300, EP300
Substrates
Histone H3, 5 μM
Acetyl CoA, 0.5 μM
Assay Properties
Reaction
[Acetyl-³H]-Acetyl Coenzyme A + histone L-lysine = Coenzyme A + [Acety-³H]-L-lysine
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| WM-8014 | >10,000 |
| WM-3835 | >10,000 |
| PF-9363 | >10,000 |
| WM-1119 | >10,000 |
| Anacardic Acid | 35,000 |
| Garcinol | 26,000 |
| Curcumin | 34,000 |
| C646 | 3,200 |
Scientific Information
Q09472 (http://www.uniprot.org/uniprot/Q09472)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Histone Acetyltransferase Assay (HAT) Services for Drug Discovery
Reference Compound IC50 for p300
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