Activity-based P38a/MAPK14 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
p38a
Enzyme
Human p38a
HGNC
MAPK14
Synonym
p38-alpha, CSBP, CSBP1,
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | >20,000 |
| Ro 31-8220 | >20,000 |
| GW 5074 | 2,300 |
| H-89 | >20,000 |
| SB202190 | 16 |
Scientific Information
Q16539 (http://www.uniprot.org/uniprot/Q16539)
Screening Location
Malvern, USA
Reference compound IC50 for P38a/MAPK14
PDF