Activity-based p38alpha kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
p38alpha
Enzyme
Human p38alpha, wt
HGNC
MAPK14
Manning ID
p38a
Synonym
p38-alpha, CSBP, CSBP1, CSBP2, CSPB1 , EC 2.7.11.24, EXIP, MXI2, Mxi2, PRKM14, PRKM15, RK, SAPK2A, p38, p38ALPHA, MAPK14, p38alpha, P38a/MAPK14
UniProt
Q16539 (MK14_HUMAN)
Kinase Type
Protein kinase
Kinase Group
CMGC
Assay Properties
Assay Format
33PanQinaseTM
Substrates
ATF2, ATP
Reaction
ATF2 + 33P-ATP = 33P-ATF2 + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Sorafenib | 540 |
| Sunitinb | 100000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | p38alpha |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – p38alpha |
| Product Number | 0443-0000-3 |
| Web Shop | Reaction Biology Online Shop |
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