Activity-based P38b/MAPK11 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
P38b/MAPK11
Enzyme
Human p38b/MAPK11
HGNC
MAPK11
Synonym
p38-beta, P38BETA2, PRKM11, SAPK2
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | >20,000 |
| Ro 31-8220 | >20,000 |
| GW 5074 | 15,000 |
| SB202190 | 62 |
| Rottlerin | >20,000 |
Scientific Information
Q15759 (http://www.uniprot.org/uniprot/Q15759)
Screening Location
Malvern, USA
Reference compound IC50 for P38b/MAPK11
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