P38g/MAPK12 Kinase Assay Service

Activity-based P38g/MAPK12 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
P38g/MAPK12

Enzyme
Human P38g/MAPK12

HGNC
MAPK12

Synonym
p38-gamma, ERK3, ERK5, ERK6

Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 200
Ro 31-8220 >100,000
GW 5074 10,600
H-89 >100,000
Rottlerin 36,000
Scientific Information
P53778 (http://www.uniprot.org/uniprot/P53778)

Screening Location
Malvern, USA

Reference compound IC50 for P38g
Reference compound IC50 for P38g

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