Activity-based p38gamma kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
p38gamma
Enzyme
Human p38gamma, wt
HGNC
MAPK12
Manning ID
p38g
Synonym
p38-gamma, ERK-6, ERK3, ERK5, ERK6, P38GAMMA, PRKM12, SAPK-3, SAPK3, p38gamma, MAPK12, P38g/MAPK12
UniProt
P53778 (MK12_HUMAN)
Kinase Type
Protein kinase
Kinase Group
CMGC
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-IRStide, ATP
Reaction
RBER-IRStide + 33P-ATP = 33P-RBER-IRStide + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 180 |
| Sorafenib | 10000 |
| Sunitinb | 100000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | p38gamma |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – p38gamma |
| Product Number | 0958-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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