Activity-based P70S6Kb/RPS6KB2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
p70S6Kb/RPS6KB2
Enzyme
Human p70S6Kb/RPS6KB2
HGNC
RPS6KB2
Synonym
KLS, P70-beta-1, P70-beta-2, S6K-beta2, S6K2, SRK, p70S6Kb, S6K-beta
Substrates
Peptide substrate, [KKRNRTLTK], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 4.9 |
| Ro 31-8220 | 38 |
| GW 5074 | 14,000 |
| H-89 | 1,400 |
| Rottlerin | 12,000 |
Scientific Information
Q9UBS0 (http://www.uniprot.org/uniprot/Q9UBS0)
Screening Location
Malvern, USA
Reference compound IC50 for p70S6Kb/RPS6KB2
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