Activity-based PAK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PAK2
Enzyme
Human PAK2
HGNC
PAK2
Synonym
PAK65, PAKgamma
Substrates
Peptide substrate, [RRRLSFAEPG], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 2.6 |
| Ro 31-8220 | 6,800 |
| GW5074 | 35,000 |
| H-89 | 39,000 |
| Rottlerin | >100,000 |
Scientific Information
Q13177 (http://www.uniprot.org/uniprot/Q13177)
Screening Location
Malvern, USA
Reference compound IC50 for PAK2
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