PAK3 Kinase Assay Service

Activity-based PAK3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
PAK3

Enzyme
Human PAK3

HGNC
PAK3

Synonym
CDKN1A, MRX30, OPHN3, PAK3beta, bPAK

Substrates
Peptide substrate, [RRRLSFAEPG], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 0.4
Ro 31-8220 5,300
GW 5074 >100,000
H-89 28,000
Rottlerin >100,000
Scientific Information
O75914 (http://www.uniprot.org/uniprot/O75914)

Screening Location
Malvern, USA

Reference compound IC50 for PAK3
Reference compound IC50 for PAK3