Activity-based PAK7 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
PAK7
Enzyme
Human PAK7, wt
HGNC
PAK5
Manning ID
PAK5
Synonym
KIAA1264, MGC26232, PAK7, PAK5
UniProt
Q9P286 (PAK5_HUMAN)
Kinase Type
Protein kinase
Kinase Group
STE
Assay Properties
Assay Format
33PanQinaseTM
Substrates
bio-4xCHKT, ATP
Reaction
bio-4xCHKT + 33P-ATP = 33P-bio-4xCHKT + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 11 |
| Sorafenib | 10000 |
| Sunitinb | 16000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | PAK7 |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – PAK7 |
| Product Number |
0410-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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