Activity-based PARP2 assay for compound screening and profiling via NAD/NADH-GloTM.
Target Overview
CAT#
PARP2
Enzyme
Human PARP2
Synonym
Poly [ADP-ribose] polymerase 2, ADP-ribosyltransferase diphtheria toxin-like 2, NAD(+) ADP-ribosyltransferase 2, Poly[ADP-ribose] synthase 2, ADPRT2, ADPRTL2
Scientific Information
Q9UGN5 (http://www.uniprot.org/uniprot/Q9UGN5)
Assay Properties
Substrate
Protein substrate, Histone H3.3, 5 µM
Activated DNA, 10 µg/ml
NAD+ (Nicotinamide Adenine Dinucleotide), 500 nM
Reaction
Substrate + NAD+ = (ADP-D-ribosyl)-Substrate + Nicotinamide
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Olaparib (AZD2281) | 0.6 |
| Veliparib (ABT-888) | 4.9 |
| Niraparib (MK-4827) | 6.6 |
| NVP-TNKS656 | 30690 |
| MN-64 | 3990 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Poly (ADP-ribose) Polymerase (PARP) Assay Services.
Reference compound inhibition for PARP2
PDF