PDE1A Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#
PDE1A
Enzyme
Human PDE1A
Synonym
Calcium/calmodulin-dependent 3′,5′-cyclic nucleotide phosphodiesterase 1A, 61 kDa Cam-PDE, hCam-1)
Substrate
1 µM cGMP, Ca-CaM
Assay Properties
Reaction
Nucleoside 3′,5′-cyclic phosphate + H₂O = nucleoside 5′-phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| IBMX | 880 |
| Methoxymethyl-IBMX | 2,030 |
| Roliptam | >50,000 |
| Methoxyquinazoline | 12,500 |
Scientific Information
P54750 (http://www.uniprot.org/uniprot/P54750)
Screening Location
Malvern, USA
Reference Compound IC50 for PDE1A
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